Abcam, ab180532, Anti-ISCU antibody [OTI4F5]

Size: 100µL
Mouse Monoclonal ISCU antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ISCU.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:OTI4F5,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human ISCU.Q9H1K1

Product details:
Clone OTI4F5 (formerly 4F5).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified from cell culture supernatant by affinity chromatography., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ISCU also known as the iron-sulfur cluster scaffold protein is an important player in the assembly of iron-sulfur clusters. This protein possesses a molecular mass of approximately 14 kDa and is widely expressed in mitochondria highlighting its central involvement in cellular processes dependent on these organelles. ISCU interacts with several proteins to facilitate the transfer of iron-sulfur clusters to apoproteins ensuring the proper maturation of these metalloproteins necessary for a variety of cellular functions.
Biological function summary
ISCU protein functions as an important scaffold component in the biosynthesis of iron-sulfur (Fe-S) clusters which are integral for the activity of multiple enzymatic and non-enzymatic systems. Within the mitochondria ISCU forms part of a multi-component complex that includes other scaffold proteins and necessary enzymes such as cysteine desulfurase which provides sulfur for cluster assembly. This complex orchestrates the precise coordination of metal ions and scaffold protein interactions facilitating cluster formation.
Pathways
The iron-sulfur cluster scaffold ISCU is vital in the electron transport chain and the citric acid cycle both of which are central to energy production in cells. ISCU closely interacts with proteins like frataxin which delivers iron to the scaffold and ferredoxin which is involved in electron transfer. These interactions highlight the pathway interdependence that maintains cellular bioenergetics and redox balance.
Disruptions in ISCU expression or function link to conditions such as Myopathy with exercise intolerance and Friedreich’s ataxia. Altered iron-sulfur cluster biogenesis impairs energy metabolism affecting muscle and neural tissues. ISCU's partnership with the frataxin protein becomes significant here as frataxin mutations result in similar pathological features thereby providing a potential target for therapeutic intervention in related diseases.