Abcam, ab180618, Anti-XPA antibody

Size: 100µL
Rabbit Polyclonal XPA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human XPA.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human XPA.P23025

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The XPA (xeroderma pigmentosum group A) protein also known as DNA repair protein complementing XP-A cells is a vital player in the nucleotide excision repair (NER) machinery. It has a molecular mass of approximately 31 kDa. XPA functions as a DNA-binding protein and is expressed in various tissues including skin cells. XPA recognizes and stabilizes the DNA damage site acting as a scaffold to recruit other repair factors ensuring the proper assembly of essential components in the repair complex.
Biological function summary
The protein is essential for maintaining genomic stability by facilitating the repair of DNA lesions. XPA operates within a multiprotein complex that includes the proteins ERCC1 and XPF. The complex identifies a wide range of structural abnormalities in DNA such as UV-induced photoproducts and chemically induced adducts. Without XPA the NER system's efficiency decreases leaving cells vulnerable to genotoxic stresses causing mutations and damage accumulation.
Pathways
XPA plays a role in the nucleotide excision repair and the base excision repair pathways. These pathways work jointly to ensure the removal of damaged DNA sections and accurate DNA synthesis. Within the NER pathway XPA interacts with proteins like RPA (Replication Protein A) and TFIIH (Transcription Factor IIH) to mediate the excision of damaged oligonucleotides. Its activity is critical for preserving the integrity of the genetic code especially in response to DNA damage caused by external agents like UV radiation.
Mutations or deficiencies in XPA are linked to xeroderma pigmentosum a disorder characterized by extreme sensitivity to ultraviolet light and a heightened risk of skin cancer. This condition results from impaired DNA repair leading to the accumulation of unrepaired DNA damage. XPA also interacts with other NER proteins like ERCC1 and XPF whose dysfunction is implicated in similar DNA repair disorders emphasizing the interconnected nature of these repair pathways in protecting against genotoxic agents.