Abcam, ab181340, Anti-PIWIL2 antibody

Size: 100µg
Rabbit Polyclonal PIWIL2 antibody. Suitable for ICC, WB, ICC/IF and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human PIWIL2.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PIWIL2. The exact immunogen used to generate this antibody is proprietary information.Q8TC59,
Specificity:At least three isoforms of PIWIL2 are known to exist; ab181340 will only detect the two longest isoforms. ab181340 is predicted to not cross-react with other PIWI protein family members.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIWIL2 also known as piwi-like RNA-mediated gene silencing 2 is a protein with a mass of approximately 98 kDa. This protein belongs to the PIWI subfamily of Argonaute proteins and is expressed across various tissues including testis and several somatic cells. PIWIL2 plays an important role in regulating gene expression through the RNA interference pathway engaging in the formation of piRNA complexes that facilitate the silencing of transposons especially in spermatogenesis.
Biological function summary
PIWIL2 functions to maintain genomic integrity and stem cell self-renewal by interacting with piRNA to suppress the activity of mobile genetic elements. It forms a ribonucleoprotein complex that is necessary for piRNA processing and function. This complex formation is vital in the regulation of transposable elements influencing processes like germ cell development and stem cell maintenance.
Pathways
PIWIL2 participates in both the RNA interference and piRNA pathway impacting gene regulatory networks critical for cell division and differentiation. In the piRNA pathway PIWIL2 associates with other proteins such as MILI and MIWI to ensure correct piRNA maturation and function. The presence of PIWIL2 in these pathways is non-redundant and important for facilitating silencing mechanisms that protect genome stability.
Altered PIWIL2 expression or function links to several types of cancer including testicular and breast cancer. PIWIL2’s interaction with other cancer-related proteins like p53 may influence tumorigenesis through the modulation of gene silencing. This association highlights its potential as a biomarker for cancer prognosis and as a target for therapeutic intervention.