Abcam, ab181640, Anti-Firefly Luciferase antibody

Size: 100µg
Anti-Firefly Luciferase antibody (ab181640) is a goat polyclonal antibody detecting Firefly Luciferase in Western Blot . Suitable for Firefly . - Over 10 publications
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Firefly,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Firefly Luciferin 4-monooxygenase.P08659,
Specificity:No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.

Product details:
What is this antibody validated in?
Anti-Firefly Luciferase antibody (ab181640) is a goat polyclonal antibody and is validated for use in Western Blot (WB) in Firefly samples.
What is the molecular weight of Firefly Luciferase?
Anti-Firefly Luciferase (ab181640) specifically detects a band for Firefly Luciferase (UniProt: P08659) at a molecular weight of 61kDa.
Trusted by the scientific community
Anti-Firefly Luciferase (ab181640) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals.

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Purification technique-Ion exchange chromatography, Purification notes-This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer., Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Firefly luciferase also known as luc assay or luciferase protein is an enzyme that catalyzes the oxidation of luciferin resulting in bioluminescence. This protein has a molecular mass of approximately 61 kDa and is responsible for the characteristic glow of fireflies. The luciferase expression occurs mainly in the light-producing organs of fireflies such as in the insect Photinus pyralis where it plays an important role in light emission.
Biological function summary
Firefly luciferase converts chemical energy into light making it valuable for various biological applications as a reporter gene. It participates in the formation of a complex with its substrates luciferin and ATP resulting in the production of a photon. This enzyme is widely used in molecular and cellular biology experiments to monitor gene expression and study protein-protein interactions due to its luminescent properties.
Pathways
Firefly luciferase functions in the bioluminescent pathway involving the conversion of luciferin to oxyluciferin and light emission. It interfaces with ATP a universal energy carrier which highlights its integration into the cellular energy systems. Firefly luciferase is similar to other oxidative enzymes though it is not directly involved with metabolic pathways like the TCA cycle or glycolysis.
Researchers often utilize firefly luciferase as a tool in tumor studies or infectious disease models. Its luminescence aids in imaging and tracking biological processes in real-time. While firefly luciferase itself is not directly associated with specific diseases the use of firefly agents allows researchers to study disease outcomes assess therapeutic efficacy and understand gene expression profiles related to cancer and viral infections often in connection with proteins such as p53 in cancer research.