Abcam, ab181842, Anti-USP9Y antibody [EPR13966]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal USP9Y antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13966,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The immunogen of USP9Y has 78% homology with USP9X (Uniprot: Q93008), which is a homologue of USP9Y on the X chromosome. In female-derived HeLa cells, we found a band which mostly likely to be USP9X. Therefore, this antibody may cross-react with USP9X.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
USP9Y also known as ubiquitin-specific peptidase 9 Y-linked is an enzyme that belongs to the family of deubiquitinating enzymes. Mechanically it functions to remove ubiquitin from ubiquitinated proteins which regulates protein degradation and recycling. The molecular mass of USP9Y is approximately 300 kDa. This enzyme is expressed primarily in the testes playing a significant role in male reproductive tissues. It is also expressed at lower levels in other tissues including the spleen and fetal brain.
Biological function summary
USP9Y is essential for sperm development and maturation processes. As a member of a larger ubiquitin protease complex it regulates the stability and turnover of proteins critical for spermatogenesis. It ensures proper cellular function by modulating protein degradation pathways which is important in maintaining the balance of cell proliferation and differentiation within the testes. This role impacts fertility by influencing the development and health of sperm cells.
Pathways
USP9Y participates in the ubiquitin-proteasome pathway an important regulatory mechanism for protein degradation. Its activity affects interactions with other proteins within this pathway including USP9X another deubiquitinating enzyme with overlapping functions. By modulating this pathway USP9Y influences the BMP signaling pathway which is critical for cell growth and differentiation. Such regulatory roles highlight its contribution to cellular homeostasis and fertility.
Mutations or deletions in the USP9Y gene can lead to male infertility often linked to conditions like azoospermia and severe oligospermia. Aberrant function or expression of USP9Y can disturb the normal spermatogenesis process resulting in defective sperm production. Its connection with infertility may also involve interactions with proteins like VASA which is important for germ cell development. Understanding USP9Y's role in these conditions can provide insights into potential therapeutic approaches for treating infertility.