Abcam, ab182148, Anti-GARNL1 antibody [EPR13592]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal GARNL1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13592,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GARNL1 also called Rap1-GTPase activating protein 4 functions mechanically as a guanine nucleotide exchange factor (GEF). It specifically activates Rap1 a small GTPase part of the Ras family by facilitating the exchange of GDP for GTP. GARNL1 has a molecular weight of approximately 184 kDa. It is expressed in various tissues including the brain and hematopoietic cells indicating its diverse functional roles across different biological systems.
Biological function summary
The ability of GARNL1 to modify Rap1 activity influences numerous cellular functions such as cell adhesion growth and differentiation. GARNL1 does its work not as an isolated player but as part of complexes that can include other signaling molecules and scaffolding proteins. By interacting with these complexes GARNL1 contributes to the regulatory mechanisms that maintain cellular homeostasis and responsiveness to extracellular signals.
Pathways
GARNL1 facilitates signal transduction within pathways like the MAPK/ERK and integrin signaling pathways. Through these pathways GARNL1 works alongside proteins like RAF kinases and integrins contributing to processes like cell migration and proliferation. The interaction with related proteins in these pathways underlines its significance in transmitting signals from the cell membrane to the nucleus influencing gene expression and cell fate decisions.
Mutations or dysregulation of GARNL1 are linked to certain cancers and neurological disorders. In oncogenesis it relates to alterations in cell adhesion and migration potentially collaborating with proteins such as BRAF in promoting tumourigenesis. Regarding neurological conditions abnormalities in GARNL1 function can affect neural connectivity and signaling highlighting its connection to Rap1-related synaptic modulation and signaling in neurons.