Abcam, ab183366, Human TARC ELISA Kit (CCL17)

Size: 1 x 96Tests
Human TARC ELISA Kit (CCL17) is a single-wash 90-min Simplestep used to quantify Human TARC (CCL17) with a sensitivity of 78.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 78.5 pg/mL,
Range:176 - 2000 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
Human TARC ELISA Kit (CCL17) (ab183366) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TARC protein in human cell culture supernatants, serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human TARC with 78.5 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
TARC is a member of the CC chemokine group that is constitutively expressed in the thymus and is transiently produced by a variety of cell types: dendritic cells (DC), endothelial cells, keratinocytes (KC), bronchial epithelial cells and fibroblasts. TARC is a ligand for chemokine receptor CCR4, expressed on Th2 lymphocytes, basophils and natural killer cells. CCL17 binds to CCR4 to induce chemotaxis in T cells.
TARC production appears to be upregulated by TNF-α and IFN-γ synergistically through NFkB and p38 signaling pathways in Human kertinocytes. Cytokines IL-4 and TGF-β1 act to downregulate TNF-α and IFN-γ induced TARC production in Human keratinocytes. TARC (CCL17) and its receptor CCR4 are believed to play important roles in the pathogenesis of the skin diseases Atopic Dermatitis (AD), Bullous pemphigoid (BP) and Mycosis fungoides (MF).

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TARC also known as CCL17 is a chemokine with a molecular mass of approximately 8 kDa. It is mainly expressed in the thymus but also found in the lung liver and lymph nodes. CCL17 plays an important role in immune response by signaling through the CCR4 receptor. It functions as a ligand that guides the movement of T-helper 2 (Th2) cells regulatory T cells (Tregs) and other immune cells to sites of inflammation.
Biological function summary
CCL17 acts as a chemotactic agent attracting specific immune cells especially T lymphocytes to inflammatory regions. This protein does not operate as part of a larger protein complex but engages directly by binding to receptors on the target immune cells. Localized expression of CCL17 increases in conditions where enhanced Th2 cell response is needed such as allergic or autoimmune responses.
Pathways
CCL17 plays a significant role in the cell signaling pathway called the chemokine signaling pathway. Through this pathway CCL17 interacts with the CCR4 receptor which helps mediate immune cell migration and adhesion. This interaction is critical for establishing the adaptive immune response. CCL17 and CCR4 support the trafficking of Th2 cells during immune reactions and pathogen defense.
CCL17 associates with asthma and atopic dermatitis where Th2 cell recruitment and activity are heightened. In asthma increased CCL17 levels exacerbate inflammatory response in the airways. In atopic dermatitis CCL17 presence corresponds with the intensity of the skin inflammation. These conditions are linked to the overactivity of Th2 cytokines such as IL-4 (H4L3) which collaborate with CCL17 to drive allergic responses.