Abcam, ab183597, Anti-Apolipoprotein E antibody [EPR19392]

Size: 20µL / 100µL / 1mL
Anti-Apolipoprotein E antibody [EPR19392] (ab183597) is a rabbit monoclonal antibody detecting Apolipoprotein E in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19392,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, WB, Flow Cyt (Intra), ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-Apolipoprotein E antibody [EPR19392] (ab183597) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB in human, mouse, rat samples.
Anti-Apolipoprotein E antibody [EPR19392] (ab183597) has been cited over 29 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Apolipoprotein E antibody [EPR19392] (ab183597) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Apolipoprotein E antibody [EPR19392] (ab183597) has been confirmed by testing in knockout samples.
Anti-Apolipoprotein E antibody [EPR19392] (ab183597) specifically detects Apolipoprotein E (UniProt ID: P08226; Molecular weight: 34kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR19392 -
ab271944
Antibody clone EPR19392 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 488, PE, APC, HRP, Alkaline Phosphatase, Alexa Fluor® 594 (
ab300743
ab302567
ab302580
ab303050
ab303051
ab303052
ab308649
ab310350
APOE (Apolipoprotein E) is a protein that mediates lipid transport and is crucial for maintaining lipid homeostasis in the brain. The APOE gene has three primary alleles: E2, E3, and E4. The APOE4 allele significantly increases the risk of Alzheimer's disease by promoting amyloid-beta aggregation and neuroinflammation. In contrast, the APOE2 allele provides a protective effect against Alzheimer's, demonstrating the complex influence of APOE on brain health and disease.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Apolipoprotein E (ApoE) also known as apolipoprotein e or apoE is a major protein involved in lipid metabolism. It has an approximate molecular weight of 34 kDa. This protein is mainly produced in the liver and brain where it plays a critical role in transporting lipoproteins fat-soluble vitamins and cholesterol. ApoE exists in three common isoforms: ApoE2 ApoE3 and ApoE4 each having different impacts on lipid binding and metabolic processes. Scientists often use an ApoE ELISA kit to quantify this protein in various samples providing insights into its expression in health and disease.
Biological function summary
ApoE mediates the binding internalization and catabolism of these lipoprotein particles facilitating their interaction with specific cell-surface receptors such as the LDL receptor. This protein operates as part of a complex that includes various other apolipoproteins and lipid molecules. The study of mouse apoe using tools like a mouse apoe ELISA provides valuable data due to its similar physiological functions in lipid transport and metabolism.
Pathways
In the lipid metabolism pathway ApoE interacts with proteins such as the LDL receptor influencing the clearance of chylomicron remnants and VLDL from the bloodstream. In the cardiovascular disease pathway this protein impacts cholesterol levels and promotes plaques stabilization. ApoE's role in these pathways offers insights into its interaction with related proteins like apolipoprotein B and LDL receptor which are critical for maintaining lipid equilibrium.
In Alzheimer’s disease ApoE4 isoform has a higher risk factor compared to ApoE3 and ApoE2 contributing to amyloid plaque formation through interactions with amyloid precursor protein. In cardiovascular diseases ApoE abnormalities influence atherosclerosis development with ApoE-deficient models showing increased susceptibility. ApoE's links to these diseases also connect it to other key proteins such as presenilin-1 in Alzheimer's disease and apolipoprotein B in cardiovascular disorders highlighting its extensive biological impact.