Abcam, ab184950, Anti-Emi1 antibody [EPR15320]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Emi1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15320,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Emi1 also known as FBXO5 is a regulatory protein involved in cell cycle control with a molecular mass of approximately 48 kDa. It functions by inhibiting the anaphase-promoting complex/cyclosome (APC/C) an important E3 ubiquitin ligase that tags proteins for degradation during mitosis. Emi1 is expressed in a variety of tissues including those with actively dividing cells like bone marrow and lymph nodes. The regulatory role of Emi1 ensures the proper timing of mitosis preventing premature progression to anaphase.
Biological function summary
Emi1 serves as an essential cell cycle regulator by controlling the activity of the APC/C therefore maintaining genomic stability during cell division. It acts independently not as part of a larger protein complex ensuring that cyclin B and securin levels remain sufficient to hold the cell in metaphase until all chromosomes are correctly attached to the spindle apparatus. This function prevents chromosomal instability and aneuploidy which can lead to malignancies.
Pathways
Emi1 plays a critical role in the cell cycle regulatory network by interacting with the APC/C complex. By regulating the degradation of cell cycle proteins Emi1 helps maintain the balance between cell proliferation and apoptosis. The regulatory influence of Emi1 on the APC/C involves interactions with Cdc20 and Cdh1 two key activators of APC/C which ensures proper cell cycle progression and checkpoint control.
Alterations in Emi1 expression or function can contribute to the development of cancer and other proliferative disorders. Emi1 overexpression is frequently found in many cancers including breast and prostate cancer where it contributes to unchecked cell proliferation. Through its interaction with the APC/C complex aberrant Emi1 activity can disrupt normal cell cycle control mechanisms leading to tumorigenesis. The protein's role in preventing APC/C-mediated degradation also makes it a connecting factor to proteins like cyclin B linking Emi1 to processes involved in oncogenesis.