Abcam, ab184951, Anti-PHD finger protein 1/PHF1 antibody [EPR14222]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal PHF1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P, ChIC/CUT&RUN-seq and reacts with Mouse, Rat, Human samples. Cited in 17 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14222,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human, Mouse,
Applications:IP, ChIC/CUT&RUN-seq, ICC/IF, WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is specific to PHD finger protein 1, not paired helical filaments.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PHD finger protein 1 also known as PHF1 is a protein involved in chromatin remodeling. It weighs approximately 60 kDa. You may find PHF1 expressed in various human tissues including the brain heart and skeletal muscle which suggests its versatile roles across different cell types. This protein contains a plant homeodomain (PHD) finger motif which functions mechanically in binding to specific histone modifications notably those associated with methylated lysine residues.
Biological function summary
PHD finger protein 1 plays a role in regulating gene expression by modifying the chromatin structure. It acts as part of the PRC2 complex which is pivotal for depositing methyl marks on histone H3 at lysine 27 (H3K27me3). This modification results in transcriptional repression of target genes. Additionally PHF1 interacts with other chromatin regulators thereby contributing to the finely-tuned control of genetic programs essential for cellular differentiation and development.
Pathways
PHD finger protein 1 is involved in the Polycomb group (PcG) pathways along with the Wnt signaling pathway. PHF1 cooperates with other proteins like EZH2 which is an important enzymatic component of the PRC2 complex to mediate its repressive function on chromatin. This interaction and pathway association highlight its involvement in maintaining stem cell pluripotency and lineage specification during development.
PHD finger protein 1 associates with cancers notably endometrial cancer and sarcomas. Alterations in PHF1 expression or function influence the disease progression partly through its interaction with EZH2 leading to abnormal silencing of tumor suppressor genes. Investigations of PHF1 reveal its potential as a biomarker and therapeutic target connecting its modulation to disease outcomes and treatment strategies.