Abcam, ab185991, Anti-MEPCE antibody - C-terminal

Size: 100µg
Rabbit Polyclonal MEPCE antibody. C-terminal. Suitable for IP, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MEPCE aa 600 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MEPCE aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q7L2J0

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab185991 was affinity purified using an epitope specific to MEPCE immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The MEPCE protein also known as 3-methylcytosine-specific methyltransferase plays an important mechanical role in RNA processing. MEPCE has a mass of approximately 81 kDa. It adds a methyl group to the 7SK snRNA a critical step in the regulation of transcription. This protein is expressed across various tissues indicating its important functions in different cellular environments.
Biological function summary
The MEPCE protein is an important player in regulating gene expression by stabilizing the 7SK snRNA in a specific ribonucleoprotein complex. This complex controls the activity of the positive transcription elongation factor b (P-TEFb) an important factor in the transcription elongation stage of gene expression. By methylating 7SK snRNA it helps maintain the repression of P-TEFb balancing transcriptional activities.
Pathways
MEPCE works collaboratively within the RNA polymerase II transcription machinery. It participates in the regulation of transcription elongation an important pathway in gene expression. MEPCE interacts closely with other proteins in this process such as LARP7 and HEXIM1 which also associate with the 7SK snRNA to modulate P-TEFb activity.
MEPCE's role in transcription regulation links it to various regulatory diseases including certain cancers and cardiac hypertrophy. The imbalance in P-TEFb activity which MEPCE helps control can lead to unregulated cell proliferation or heart muscle enlargement. MEPCE in association with LARP7 and HEXIM1 adds a layer of complexity to these conditions as alterations in this regulatory system can provoke disease onset or progression.