Abcam, ab186003, Anti-SKA3 antibody - C-terminal

Size: 100µg
Rabbit Polyclonal SKA3 antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human SKA3 aa 350 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SKA3 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q8IX90

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SKA3 protein also known as spindle and kinetochore-associated protein 3 plays a significant role in mitosis. Its molecular weight is approximately 46 kDa. Researchers find SKA3 expressed mainly in proliferating tissues and mitotic phases of dividing cells. It interacts directly with kinetochore microtubules ensuring proper chromosome alignment and segregation during cell division.
Biological function summary
In the context of cell division SKA3 forms part of the SKA complex. This complex works with the NDC80 complex which is essential for microtubule attachment at kinetochores. SKA3 aids in stabilizing these attachments and is important for accurate chromosomal movement. Disruption in SKA3 expression or function can lead to mitotic errors which may affect overall cell viability and proliferation.
Pathways
SKA3 plays an essential role in the spindle assembly checkpoint pathway. It collaborates with protein partners like SKA1 and SKA2 and connects to the broader network of the SAC pathway that monitors cell cycle progression. SKA3 ensures that cells do not proceed to anaphase until all chromosomes are correctly aligned which is important for genomic stability.
Errors in SKA3 function relate to conditions like cancer and aneuploidy. Abnormal SKA3 expression can lead to defective cell division resulting in tumorigenesis. Mutations in SKA3 associate with other proteins like BubR1 and Aurora B which also affect the spindle checkpoint and have implications in cancer development. Correcting SKA3-related pathway dysfunctions might offer potential therapeutic strategies in these diseases.