Abcam, ab186081, Recombinant Human PPP3CA + PPP3R1 protein

Size: 25µg
Recombinant Human PPP3CA + PPP3R1 protein is a Human Full Length protein, in the 1 to 170 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, ELISA, WB, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:SDS-PAGE, WB, ELISA, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P63098,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute the contents of the vial using ultrapure water or sterile PBS to prepare a stock solution of 0.5 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 2-8ºC for up to 1 week. For extended storage, freeze in working aliquots,
Storage buffer:pH: 7Constituents: 0.88% Sodium chloride, 0.79% Tris HCl, 0.03% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Product details:
Prior to reconstitution, store the lyophilized protein at 2-8°C. After reconstitution, store in working aliquots at -80°C.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PPP3CA also known as calcineurin A alpha is a catalytic subunit of the protein phosphatase calcineurin complex which is a calcium and calmodulin-dependent serine/threonine protein phosphatase. The molecular weight of PPP3CA is approximately 61 kDa. It is highly expressed in various tissues especially in the brain heart and skeletal muscles. PPP3R1 also referred to as calcineurin B is the regulatory subunit that binds calcium ions and helps in targeting the phosphatase activity of PPP3CA. Together they form the active calcineurin complex.
Biological function summary
Calcineurin plays an important role in calcium signaling pathways by dephosphorylating target proteins therefore activating transcription factors like NFAT (nuclear factor of activated T-cells). This complex regulates several cellular functions such as gene expression particularly in immune cells and neurons. Through its influence on different proteins calcineurin impacts processes including the immune response and synaptic plasticity.
Pathways
Calcineurin is an important player in the calcium-controlled signal transduction pathway. It directly influences proteins involved in the regulation of diverse cellular responses. Particularly it activates NFAT proteins by dephosphorylation leading to their translocation into the nucleus where they regulate gene expression. Another pathway involves calcineurin’s role in modulating the activity of other substrate proteins involved in muscle differentiation and neural development relating it closely to proteins like CABIN1.
The dysregulation of calcineurin activity has been linked to cardiac hypertrophy and immunological disorders such as autoimmune diseases. Alterations in the calcineurin-associated pathways affect the normal functioning of NFAT contributing to the pathological cardiac growth observed in hypertrophy while lining to disorders in immune function might involve proteins such as AKNA. Understanding calcineurin's function and regulation remains essential for developing treatments for these conditions.