Abcam, ab186123, Anti-NMT1/NMT antibody

Size: 100µg
Rabbit Polyclonal NMT1/NMT antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human NMT1 aa 50-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NMT1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.P30419

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
N-myristoyltransferase 1 often referred to as NMT1 or NMT is an enzyme essential for catalyzing the transfer of myristate a 14-carbon saturated fatty acid to the N-terminal glycine residue of substrate proteins. This post-translational modification is called myristoylation and is necessary for the proper functioning of many proteins. NMT1 has a molecular mass of approximately 50 kDa and is widely expressed in eukaryotic cells prominently in the cytoplasm and sometimes associated with intracellular membranes.
Biological function summary
NMT1 modifies proteins by attaching myristic acid influencing their membrane-binding capability stability and function. It does not form part of a larger complex but rather acts individually to regulate protein activity through myristoylation. This modification is important for the localization and activation of proteins involved in various cellular processes including signal transduction and apoptosis.
Pathways
N-myristoyltransferase 1 plays a role in several key biological pathways. Myristoylation mediated by NMT1 regulates signal transduction pathways such as the Src family kinases and G-protein signaling. Through these pathways NMT1 interacts with key proteins like Src a tyrosine kinase involved in cell growth and differentiation and Gα proteins which are part of the G-protein coupled receptor signaling pathway.
Alterations in NMT1 activity are associated with various cancers and infectious diseases. For instance overexpression of NMT1 has been observed in colorectal cancer potentially linked through its interactions with cancer-related proteins like Src. Additionally the enzyme is important for the survival of certain pathogens which require myristoylation for their life cycle suggesting that NMT1 could be a target for antimicrobial drug development.