Abcam, ab186132, Anti-PFKP antibody

Size: 100µg
Rabbit Polyclonal PFKP antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PFKP aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PFKP aa 1-50. The exact immunogen used to generate this antibody is proprietary information.Q01813

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab186132 was affinity purified using an epitope specific to PFKP immobilized on solid support., Storage buffer-pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PFKP also known as 6-phosphofructokinase platelet type functions as an important glycolytic enzyme that catalyzes the phosphorylation of fructose 6-phosphate to fructose 16-bisphosphate using ATP. This process shows the rate-limiting step in glycolysis. The PFKP protein weighs approximately 85 kDa. Expression occurs in various tissues with high presence in platelets and muscle tissues indicating its significant role in energy metabolism.
Biological function summary
PFKP plays an essential role in maintaining energy supply ensuring efficient glycolysis during high energy demands. It associates with other isoforms PFKM (muscle) and PFKL (liver) to form a tetrameric complex which can adapt its activity based on the energy needs of the cell. This adaptability defines its contribution to cellular metabolism regulation considering energy substrates and signaling molecules.
Pathways
The function of PFKP ties closely to glycolysis and the larger metabolic pathways managing energy production such as the tricarboxylic acid (TCA) cycle. Within these pathways PFKP interacts with other metabolic regulators including AMP-activated protein kinase (AMPK) which influences cell metabolism in response to energy availability. Connections with PFKL and PFKM reveal that PFKP's activity relates to the overall glycolytic flux and energy balance.
PFKP's alterations contribute to metabolic conditions like cancer and diabetes. Its dysregulation in cancer cells supports the Warburg effect where tumor cells prefer glycolysis. The link with metabolic proteins such as AMPK also indicates a potential role in diabetes as both disorders involve shifts in energy metabolism. Understanding PFKP's impact on these diseases helps in developing therapeutic strategies targeting metabolic pathways.