Abcam, ab186435, Anti-EAAT4 antibody [EPR7742] - N-terminal

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal EAAT4 antibody. N-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR7742,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EAAT4 also known as Excitatory Amino Acid Transporter 4 or SLC1A6 functions as a sodium-dependent glutamate transporter primarily facilitating the reuptake of glutamate into presynaptic neurons and glial cells. EAAT4 has an approximate molecular mass of 65 kDa. Expression occurs mainly in Purkinje cells within the cerebellum but it can also be found in other parts of the central nervous system albeit at lower levels.
Biological function summary
EAAT4 controls extracellular glutamate concentration preventing excitotoxicity in neural circuits. EAAT4 forms part of the larger family of high-affinity glutamate transporters which includes EAAT1 to EAAT5. This transporter operates by coupling glutamate transport with the influx of sodium and the efflux of potassium and protons thereby maintaining ion balance. Although single in its action EAAT4 works alongside related transporters for comprehensive glutamate regulation.
Pathways
EAAT4 plays a significant role in glutamatergic signaling and cerebellar function. The transporter deeply integrates into the synaptic transmission pathway ensuring glutamate homeostasis critical for synaptic plasticity. EAAT4 works in functional tandem with proteins like EAAT3 facilitating efficient glutamate clearance and modulating excitatory signaling within neuronal pathways.
EAAT4 relates to neurodegenerative conditions where disrupted glutamate homeostasis plays a part such as Amyotrophic Lateral Sclerosis (ALS) and Spinocerebellar Ataxia. Excessive extracellular glutamate can lead to neuronal damage linking EAAT4 function with these pathologies. Moreover EAAT4 interacts with proteins like GLT-1 in these disease contexts to attempt to mitigate glutamatergic dysfunction.