Abcam, ab187026, Anti-RhoA + RhoC antibody [EPR18133]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal RHOA antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 5 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18133,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, Flow Cyt (Intra), IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RhoA and RhoC also known as RAS homolog family member A and C are small GTPases. They play an essential role in regulating the actin cytoskeleton which impacts cell morphology motility and division. RhoA has a molecular mass of approximately 22 kDa. RhoC shares a similar size and structure. Both are expressed in various human tissues but they show higher expression in the brain lung and liver. These proteins can switch between an active GTP-bound state and an inactive GDP-bound state influencing cellular processes.
Biological function summary
The proteins RhoA and RhoC are key regulators in cell signaling pathways that coordinate cytoskeletal dynamics. They are integral parts of the Rho protein complex which includes other small GTPases like Rac1 and Cdc42. Through these interactions RhoA and RhoC influence cell cycle progression apoptosis and cellular transformations required during development and tissue repair. Their ability to control actomyosin contractility makes them significant for focal adhesion assembly and disassembly affecting cell movement.
Pathways
The activities of RhoA and RhoC are involved in the Rho signaling pathway and the Wnt signaling pathway. These pathways play an important role in guiding cellular architecture and communication. The Rho signaling pathway includes interactions with proteins like ROCK1 and ROCK2 which help modulate smooth muscle contraction and cell trophism. Meanwhile the Wnt signaling pathway involves cross-talk with β-catenin and influences cellular proliferation and growth pointing to RhoA and RhoC’s involvement in these essential signal transduction processes.
The dysregulation of RhoA and RhoC is linked with various cancers including breast and prostate cancer where they contribute to cancer cell invasion and metastasis. RhoA is also connected to cardiovascular disorders where abnormal Rho signaling can lead to hypertension due to its effect on vascular smooth muscle contraction. In the context of cancer these proteins often work alongside others like Ras which can exacerbate cellular transformation and tumorigenesis. Understanding these connections offers opportunities for targeted therapeutic interventions in these pathological conditions.