Abcam, ab18723, Anti-Doublecortin antibody

Size: 50µg
Anti-Doublecortin antibody (ab18723) is a rabbit polyclonal antibody detecting Doublecortin in Western Blot, IHC-P, IHC-Fr, ICC/IF . Suitable for Mouse, Quail, Rat . - Over 470 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Quail,
Applications:ICC/IF, IHC-P, WB, IHC-FoFr, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Please note: Low dilutions of this antibody can cause high background in IHC. Please use as high a dilution as possible. Optimal working dilutions are batch dependent.

Product details:
Anti-Doublecortin antibody (ab18723) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-FoFr, IHC-Fr, IHC-P and WB.
Anti-Doublecortin antibody (ab18723) was first used in a scientific publication in 2004 and has been cited over 474 times in peer reviewed journals. It's performance in Western blot and IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Doublecortin antibody (ab18723) has high sensitivity and specificity.
Anti-Doublecortin antibody (ab18723) has 59 independent reviews from customers.
Anti-Doublecortin antibody (ab18723) specifically detects Doublecortin (UniProt ID: O43602; Molecular weight: 41kDa) and is sold in 50 µg selling sizes.
Doublecortin (DCX) is a critical protein in neuro research, particularly for its role in neuronal migration and neurogenesis. It is predominantly expressed in immature neurons and is essential for proper brain development. Researchers use doublecortin to study the formation and migration of new neurons, making it vital for understanding neurodevelopmental disorders and brain repair mechanisms. Its expression helps in assessing the dynamics of neuronal growth and the potential for neuroplasticity in various neurological conditions.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Doublecortin also known as DCX or Doublecortin-X is a microtubule-associated protein with a mass of approximately 40 kDa. This protein is found mainly in the developing nervous system where it plays an important role in neuronal migration. Doublecortin locates to neuroblasts and immature neurons serving as a marker for neuronal precursor cells. Its level of expression decreases as neurons mature making doublecortin staining a valuable tool in identifying neuronal progenitors during development.
Biological function summary
Doublecortin stabilizes microtubules facilitating proper neuronal migration. It does not operate alone but collaborates within microtubule-binding protein complexes to guide neurons to their destination during the cortical development process. The protein along with its isoforms like Doublecortin-Anticuerpo serves as a reliable indicator of neurogenesis impacting the cytoskeletal dynamics important for brain architecture.
Pathways
Doublecortin participates chiefly in the Reelin and L1CAM pathways which are involved in neuronal positioning and axonal growth. In these pathways doublecortin interacts with proteins like LIS1 playing a significant role in transporting molecular cargo along microtubules. These pathways are essential for the proper layering of neurons in the cerebral cortex and are highly coordinated with signaling and adaptor proteins.
Mutations in doublecortin relate directly to lissencephaly a brain malformation disorder and epilepsy. These conditions showcase a strong connection between doublecortin and the LIS1 protein both of which regulate neuronal migration. While DCX marker analysis can elucidate disease mechanisms doublecortin ELISA and other techniques help in diagnosing disorders linked to its dysfunction such as Eilrayna Gelyana a syndrome affecting brain development and function.