Abcam, ab187381, Anti-Acidic Cytokeratin antibody [AE-1]

Size: 500µL
Mouse Monoclonal Cytokeratin 10 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KRT10.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:AE-1,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human KRT10.P13645,
Specificity:ab187381 recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Acidic cytokeratins often referred to as type I keratins form an essential component of the intermediate filament family of proteins. They have a molecular mass typically around 40 to 67 kDa and are found in epithelial cells. These proteins are known for their structural roles providing mechanical support and resilience to cells. Acidic cytokeratins pair with basic cytokeratins to create the cytoskeletal framework that maintains cell integrity and shape.
Biological function summary
The family of acidic cytokeratins contributes significantly to cell differentiation migration and signal transduction. They often form complexes with basic keratins which are critical for the stability and function of keratin networks. In various epithelial tissues these complexes protect cells against mechanical stress and participate in dynamic cellular processes like wound healing and epithelial repair.
Pathways
Acidic cytokeratins participate in cellular pathways related to the cytoskeleton and cell adhesion such as the Wnt and MAPK signaling pathways. These pathways influence epithelial cell behavior and interaction with their microenvironment. Related proteins in these pathways include beta-catenin which interacts with cytoskeletal components during signal transduction and cell junction remodeling.
Irregular expression of acidic cytokeratins associates with several cancers including breast and colorectal cancer. These proteins can serve as biomarkers for tumor diagnosis and progression. Additionally mutations in acidic cytokeratins link to skin diseases like epidermolysis bullosa simplex a condition disrupting normal skin structure. Proteins such as p53 which are involved in cell cycle regulation may also connect with acidic cytokeratins in the context of these diseases highlighting their significance in diagnostic and therapeutic research.