Abcam, ab187400, Rabbit IgG ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests / 10 x 96Tests
Rabbit IgG ELISA Kit is a single-wash 90-min Simplestep used to quantify Rabbit IgG with a sensitivity of 0.23 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput - Cited in over 5 citations
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Rabbit,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.23 ng/mL,
Range:0.31 - 20 ng/mL,
Assay time:1h 30m,
Assay Platform:Microplate

Product details:
Rabbit IgG ELISA Kit ab187400 is a rapid single-wash 90-min Sandwich ELISA to measure Rabbit IgG in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 0.23 ng/mL.
How the assay works
Rabbit IgG SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Rabbit IgG ELISA Kit ab187400 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Save your precious samples
Our 384-well Rabbit IgG ELISA Kit only requires a maximum of 12.5µL sample
There are five classes of immunoglobulins in Human and mouse: IgA, IgD, IgE, IgM, and IgG. So far, four istotypes have been identified (IgA, IgE, IgG, and IgM) in rabbit. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue. Rabbit has only one IgG subclass,The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves which together form a Y like structure. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane. IgG is involved in response to a foreign antigen. The presence of IgG usually signifies a mature antibody response. IgG has a molecular weight of about 150 kDa, it can bind to many pathogens and also plays an important role in antibodydependent cell-mediated cytotoxicity. Typically rabbit serum and plasma samples contain about 5 to 10 mg/ml of IgG. The rabbit immune system has been documented as a vehicle for developing antibodies with higher affinity and more diverse recognition of many molecules including phospho-peptides, carbohydrates and immunogens that are not otherwise immunogenic in mouse. The main type of antibodies available from rabbit is polyclonal antibodies. In recent years, the use of hybridoma technology for the production of rabbit monoclonal antibodies has created the need for a fast and simple procedure for quantifying antibody production in vitro (e.g., culture supernatant) or in vivo (e.g., ascites). An accurate determination of immunoglobulin levels in the serum, plasma or hybridoma-culture supernatant is essential to study the effect of drugs or physical parameters on the level of immunoglobulin secretion or hybridoma growth.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C