Abcam, ab187660, Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal PKN3 phospho T718 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR1044(2),
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein Kinase C-related kinases (PRK1 PRK2 and PRK3) are serine/threonine kinases involved in signal transduction. PRK1 also referred to as PKN1 has a molecular mass of approximately 98 kDa. These kinases are generally expressed in tissues like the brain heart and skeletal muscle. PRK2 also known as PKN2 and PRK3 sometimes referred to as PKN3 share similar structural features having conserved regions like the catalytic and regulatory domains. All three kinases play roles in cytoskeletal dynamics and cellular signaling.
Biological function summary
PRK1 PRK2 and PRK3 act as downstream effectors of Rho GTPases which also suggests they can be involved in a complex. These kinases regulate actin cytoskeleton organization affecting cell shape and motility. PRK1 participates in processes like cell cycle progression and differentiation while PRK2 influences membrane trafficking and protein translation. PRK3 although less studied is similar in structure suggesting potential overlapping roles in cellular functions.
Pathways
PRK1 PRK2 and PRK3 integrate into the RhoA signaling pathway playing a part in actin remodeling. In particular PRK2 links closely to the mTOR pathway associated with cell growth and protein synthesis regulation. Through these pathways these kinases interact with other proteins such as Rho GTPases and mTORC1 coordinating a variety of critical cellular tasks and signaling events.
Aberrant regulation of PRK1 PRK2 and PRK3 can connect to cancer and cardiovascular diseases. Overexpression or mutations of these kinases associate with various cancers promoting uncontrolled growth and metastasis. PRK2 particularly shows a relation to cardiac hypertrophy due to its role in heart muscle regulation. These kinases interact with key proteins like Akt and RhoA which further links them to oncogenic signaling and heart disease pathways.