Abcam, ab188010, Anti-DDX49 antibody

Size: 100µL
Rabbit Polyclonal DDX49 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Probable ATP-dependent RNA helicase DDX49 aa 350-450.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human Probable ATP-dependent RNA helicase DDX49 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.Q9Y6V7

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DDX49 also known as DEAD-box helicase 49 is an RNA helicase that is involved in the unwinding of RNA secondary structures an important step in the regulation of various RNA metabolic processes. It has a mass of approximately 75 kDa and is expressed in multiple tissues with higher expression noted in certain tissues. This protein belongs to the DEAD-box family characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). DDX49 is commonly located in the nucleus where it plays a critical role in RNA processing events.
Biological function summary
DDX49 functions as a critical regulator in the process of ribosome biogenesis where it facilitates the assembly and maturation of ribosomal RNA (rRNA) within the nucleolus. It operates as part of a larger ribonucleoprotein complex contributing to the correct folding and processing of rRNA precursors into mature rRNA species. This protein’s helicase activity is essential for ensuring the accurate assembly of ribosome components directly affecting cellular protein synthesis.
Pathways
DDX49 plays a role in the ribosomal biogenesis pathway and the broader RNA processing pathway. These pathways are essential for maintaining normal cellular growth and proliferation. Within these pathways DDX49 interacts with other proteins like nucleolin (NCL) and fibrillarin (FBL) which are integral to the ribosome assembly process. The interactions between these proteins help regulate the proper production and function of ribosomes in cells.
Alterations in DDX49 have been implicated in specific cancers such as colorectal cancer and leukemia where dysregulation in RNA metabolism can disrupt normal cellular function. In these cancers DDX49 may cooperate with oncogenic factors like c-Myc a well-studied transcription factor that also influences ribosomal RNA synthesis and ribosome biogenesis. Understanding the role of DDX49 in these contexts may provide insights into new therapeutic strategies targeting ribosome biogenesis in cancer.