Abcam, ab188171, Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Eph receptor A5/BSK phospho Y779 + Y833 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5684,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody only detects EPH A3 phosphorylated at Tyrosine 779, EPH A4 phosphorylated at Tyrosine 779, and EPH A5 phosphorylated at Tyrosine 833.

Product details:
ab188171 is the carrier-free version of
ab124881
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EPH receptors A3 A4 and A5 which are also known as EPHA3 EPHA4 and EPHA5 are part of the receptor tyrosine kinase family. These proteins have a mass around 100-130 kDa. They predominantly express in neural tissues but one can also find them in certain epithelial and mesenchymal tissues. Functionally they play a role in cell-cell communication by binding with ephrin ligands which leads to kinase activation and downstream signaling cascades.
Biological function summary
EPH receptors A3 A4 and A5 are essential for mediating a range of cellular processes including cell migration axon guidance and tissue boundary formation. They do not act in isolation but often form complexes with ephrin ligands such as ephrin-A1 and ephrin-A5. These interactions are critical in developmental processes and maintaining cellular architecture. By influencing cell positioning and movement they significantly contribute to nervous system development and vascular patterning.
Pathways
EPH A3 A4 and A5 integrate into the ephrin signaling pathway and the integrin-mediated signaling pathway. In the ephrin signaling pathway they work in tandem with related proteins such as EPHA2 and EPHB receptors to modulate cellular dynamics. These pathways dictate cytoskeletal organization influencing cellular adhesion and motility impacting a variety of physiological processes including angiogenesis and nerve development.
Altered functions of EPH A3 A4 and A5 associate with cancer and neurodegenerative diseases. In cancer especially glioblastoma and melanoma their overexpression or aberrant activity can lead to enhanced cell proliferation and metastasis partially through interactions with proteins like EPHA2. In neurodegenerative conditions such as Alzheimer's disease dysregulated EPH receptor signaling may contribute to neuronal death and cognitive impairment connected with proteins involved in tau pathologies.