Abcam, ab189173, Anti-TRC8 antibody - C-terminal

Size: 50µg
Goat Polyclonal TRC8 antibody. C-terminal. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RNF139 aa 650 to C-terminus.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RNF139 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q8WU17

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: 99% Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRC8 also known as RNF139 or RING finger protein 139 is an E3 ubiquitin ligase that plays a role in protein degradation through ubiquitination. TRC8 has a mass of approximately 88 kDa. It is a transmembrane protein found in the endoplasmic reticulum. The presence of its RING finger domain aids in transferring ubiquitin to substrate proteins marking them for proteasomal degradation. TRC8 is expressed in multiple tissues and cells including the liver and prostate suggesting various functional roles.
Biological function summary
TRC8 is involved in the regulation of protein quality control by mediating the degradation of defective proteins within the endoplasmic reticulum. As part of the complex machinery of the ubiquitin-proteasome system TRC8 oversees protein homeostasis assisting in the removal of misfolded or unnecessary proteins to maintain cellular balance. This function impacts several cellular processes and maintains the health of the cell under stress conditions.
Pathways
The TRC8 protein interacts with components of the endoplasmic reticulum-associated degradation (ERAD) pathway which is a critical process for maintaining protein balance. TRC8 works alongside other proteins like HRD1 and SEL1L in this pathway to ensure the clearance of defective proteins. It also influences the sterol regulatory element-binding protein (SREBP) pathway impacting cholesterol synthesis and homeostasis.
The role of TRC8 is significant in cancer and genetic syndromes. Mutations or deletions in the TRC8 gene associate with hereditary leiomyomatosis and renal cell cancer (HLRCC) where its interaction with proteins like fumarate hydratase is important. It is also implicated in neurodegenerative disorders where dysfunctional ubiquitin ligase activity impacts neuronal protein homeostasis potentially linked through connections with proteins like Parkin in managing protein aggregations.