Abcam, ab190103, Anti-SCAP antibody

Size: 200µL
Rabbit Polyclonal SCAP antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human SCAP aa 600-650 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WB, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SCAP aa 600-650 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.Q12770

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SCAP also known as SREBP cleavage-activating protein is an important player in lipid homeostasis. SCAP has a molecular mass of approximately 150 kDa. It locates in the endoplasmic reticulum and Golgi apparatus within cells. The protein facilitates the movement of sterol regulatory element-binding proteins (SREBPs) from the endoplasmic reticulum to the Golgi. This action activates SREBPs by enabling their cleavage which is necessary for cholesterol and lipid synthesis regulation.
Biological function summary
SCAP acts as a cholesterol sensor when paired with SREBPs in a complex. The SCAP-SREBP complex remains inactive within the endoplasmic reticulum at high cholesterol concentrations. When cholesterol levels decrease SCAP aids in the transport of SREBPs to the Golgi apparatus. This transport triggers proteolytic activation of SREBPs which then migrate to the nucleus to promote gene expression for lipid metabolism.
Pathways
SCAP is instrumental in the cholesterol biosynthesis and lipid metabolic pathways. The protein interacts closely with SREBPs in these pathways to regulate lipid balance within cells. In particular SCAP's action is linked with the insulin signaling pathway aiding in the coordination of growth factor-mediated lipid synthesis. This highlights its importance in the control of lipid-related gene transcription together with proteins like Insig which anchor the SCAP-SREBP complex under high cholesterol levels.
SCAP plays a role in metabolic syndrome and cardiovascular diseases. Dysregulation of SCAP can lead to abnormal cholesterol levels contributing to atherosclerosis and other lipid disorders. The function of SCAP connects to proteins like HMG-CoA reductase in these conditions as it co-regulates the synthesis of cholesterol. Understanding SCAP's role in these diseases may open new therapeutic strategies for managing cholesterol-related disorders.