Abcam, ab190361, Anti-WDR77 antibody [EPR10709(2)] - C-terminal

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal WDR77 antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10709(2),
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, IP, ICC/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
WDR77 also known as MEP50 is a protein with a mass of about 37 kDa. It functions as a cofactor involved in methylation processes often forming part of protein complexes with PRMT5. WDR77 locates within the cell nucleus and is widely expressed in human tissues with higher levels observed in reproductive organs like the testis and prostate. The protein interacts with other proteins to affect transcription and RNA processing.
Biological function summary
WDR77 interacts with the protein arginine methyltransferase 5 (PRMT5) and contributes to the assembly and stabilization of methyltransferase complexes. This role is essential for its participation in the methylation of arginine residues on histones and other proteins which regulates gene expression. WDR77 influences cell proliferation and differentiation by modulating these processes especially in germ cells and prostate epithelial cells.
Pathways
WDR77 is an important player in the transcriptional repression pathway affecting histone modification. It connects intimately with important pathways like the PI3K/AKT signaling and cell cycle regulation. Through its interaction with PRMT5 WDR77 takes part in regulatory networks impacting gene silencing chromatin remodeling and the overall gene expression mechanism. The involvement of WDR77 in these pathways emphasizes its relationship with PRMT5 and other methylation-associated proteins.
WDR77 has links to prostate cancer and lung cancer. These associations may result from its role in cell cycle control and proliferation which becomes dysregulated in oncogenesis. WDR77's interaction with PRMT5 is critical in such conditions as changes in their activity can alter normal cell growth patterns. Research indicates that abnormal expression or function of WDR77 can lead to increased tumorigenesis in specific tissues emphasizing the protein's potential as a target for therapeutic interventions.