Abcam, ab190542, Monkey Cystatin C ELISA Kit

Size: 1 x 96Tests
Monkey Cystatin C ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Monkey Cystatin C in Monkey in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Monkey,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.3303 ng/mL,
Range:0.125 - 4 ng/mL,
Assay Platform:Microplate

Product details:
Abcam's Cystatin C Monkey ELISA Kit (ab190542) is for the measurement of Cystatin C in monkey serum and plasma.
In this assay the Cystatin C present in samples reacts with the anti-Cystatin C antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-Cystatin C, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Cystatin C in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Cystatin C in the test sample. The quantity of Cystatin C in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Cystatin C is a small cysteine proteinase inhibitor present in body fluids. Studies have shown Cystatin C levels to be directly correlated with the glomular filtration rate.
The calibrator used in this kit is of human origin.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols