Abcam, ab190546, Sheep IgG ELISA Kit

Size: 1 x 96Tests
Sheep IgG ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Sheep IgG in Sheep in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Sheep,
Assay type:Sandwich (quantitative),
Sensitivity:= 4.28 ng/mL,
Range:15.625 - 500 ng/mL,
Assay Platform:Microplate

Product details:
Abcam's IgG Sheep ELISA Kit (ab190546) is a two-site enzyme linked immunoassay (ELISA) for the measurement of IgG in sheep biological samples.
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols