Abcam, ab191385, Anti-SIRT6 antibody [EPR18463]

Size: 20µL / 100µL / 1mL
Anti-SIRT6 antibody [EPR18463] (ab191385) is a rabbit monoclonal antibody detecting SIRT6 in Western Blot, IP, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 30 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18463,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-SIRT6 antibody [EPR18463] (ab191385) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of SIRT6?
Anti-SIRT6 [EPR18463] (ab191385) specifically detects a band for SIRT6 (UniProt: Q8N6T7) at a molecular weight of 39kDa.
Trusted by the scientific community
Anti-SIRT6 [EPR18463] (ab191385) was first used in a scientific publication in 2016 and has been cited over 30 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Specificity confirmed
The specificity of Anti-SIRT6 antibody [EPR18463] (ab191385) has been confirmed by Western blot testing in SIRT6 Knockout HeLa cell line,
ab265054
Other related products
We have a range of other formats of antibody clone [EPR18463] also available for your convenience: ab191385, Alexa Fluor® 647 -
ab225363
, Alexa Fluor® 488 -
ab225364
, Carrier free -
ab236024
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SIRT6 protein also known as Sirtuin 6 plays an important role in cellular regulation. It is a member of the sirtuin family of proteins which are NAD+-dependent deacetylases. SIRT6 weighs approximately 39 kDa and can be found in the nucleus of many cell types. It acts mainly in regulating cellular homeostasis by removing acetyl groups from histone proteins which affects gene expression. Its expression levels vary across different tissues and it is known to be active in metabolically active organs such as the liver and brain.
Biological function summary
SIRT6 influences DNA repair metabolism and inflammation. It participates in maintaining genomic stability by promoting base excision repair a critical DNA repair process. Moreover SIRT6 contributes to glucose homeostasis by influencing gluconeogenesis and glycolysis. This protein is not known to be part of any larger protein complexes but it interacts individually with other proteins to exert its biological effects.
Pathways
SIRT6 plays a significant role in two key biological pathways: DNA damage response and metabolism regulation. In the DNA damage response pathway SIRT6 works with other proteins like PARP1 to facilitate DNA repair under stress conditions. In the regulation of metabolism SIRT6 interacts with transcription factors like HIF1α which influences the expression of genes involved in glycolytic metabolism and glucose homeostasis.
SIRT6 has associations with cancer and aging-related diseases. SIRT6 has a protective effect against oncogenesis by maintaining genomic stability and regulating metabolic pathways that cancer cells exploit. Additionally its role in aging is connected to its ability to prevent age-related genomic instability and metabolic decline. In cancer SIRT6 interacts with proteins such as c-Myc and p53 influencing cell growth and apoptosis pathways. This highlights its potential as a therapeutic target for both cancer and age-related conditions.