Abcam, ab191598, Anti-RNA polymerase beta antibody [EPR18704]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal RNA polymerase beta antibody. Suitable for WB, IP and reacts with Escherichia coli samples. Cited in 14 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18704,
Isotype:IgG,
Carrier free:No,
Reacts with:Escherichia coli,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNA polymerase beta also known as RpoB or DNA-directed RNA polymerase subunit beta is a critical enzyme in the transcription process. This protein has a molecular mass of approximately 150 kDa. RpoB plays a role by catalyzing the synthesis of RNA from a DNA template an essential step in gene expression. It is widely expressed in bacteria mitochondria chloroplasts and also in the nuclei of eukaryotic cells. RpoB functions as a fundamental part of the core RNA polymerase complex which also includes other subunits such as alpha beta' and omega.
Biological function summary
RpoB interacts with multiple subunits to form the RNA polymerase holoenzyme integrating into cellular machinery to initiate transcription. This enzyme participates in synthesizing mRNA rRNA and tRNA all of which are important for protein synthesis. The holoenzyme formation aligns with promoter regions on DNA enabling precise transcription initiation. Without the proper function of RpoB and its associated subunits transcription efficiency and fidelity could be compromised impacting cell viability.
Pathways
RNA polymerase beta is integral to transcriptional regulation pathways and genetic response mechanisms within the cell. It plays a role in the bacterial transcription pathway directly interacting with sigma factors and other transcription regulators to manage gene expression in response to environmental changes. Furthermore RpoB operates in conjunction with other proteins like sigma factors which allow the enzyme to recognize specific promoter sequences ensuring the transcription of appropriate genes.
The improper function of RNA polymerase beta has correlations with bacterial resistance to antibiotics notably rifampicin. Mutations in the rpoB gene can lead to resistance complicating treatment strategies for conditions like tuberculosis. This enzyme's relationship with sigma factors also presents a route through which these mutations affect transcription fidelity leading to resistant bacterial strains. Understanding these connections helps to develop therapies targeting the bacterial transcription machinery effectively.