Abcam, ab192234, Anti-Nesprin1/Syne-1 antibody [EPR14196]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Nesprin1/Syne-1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 20 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14196,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody is not suitable for using in tissue samples when testing in WB application.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nesprin1 also known as Syne-1 is a large spectrin repeat protein with a mass of approximately 1002 kDa. It is part of the plakin family of proteins and plays an important role in linking the nuclear envelope to the actin cytoskeleton. Nesprin1 is widely expressed in skeletal and cardiac muscle tissues where it helps maintain nuclear positioning and structural integrity. This protein has several isoforms resulting from alternative splicing which allows it to perform diverse functions in different cellular contexts.
Biological function summary
Nesprin1 acts as an important component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex. The LINC complex connects the nuclear envelope to the cytoskeleton facilitating mechanical signal transduction and cellular communication. Nesprin1's interactions with other elements of the cytoskeleton and nuclear envelope help regulate nuclear anchoring and positioning essential for cellular dynamics and function. In muscle cells Nesprin1 stabilizes the nucleus and synchronizes cellular responses to mechanical stress.
Pathways
Nesprin1 participates in nuclear-cytoskeletal coupling pathways that are vital for cellular biomechanics. It associates with proteins such as SUN1 and SUN2 within the LINC complex to mediate structural continuity between the nucleus and the cytoskeleton. Furthermore it plays a role in the mechanotransduction pathway linking external mechanical signals to gene expression changes. This connection is significant for maintaining cellular homeostasis and adaptability in response to mechanical stress.
Nesprin1 mutations are associated with muscular dystrophies particularly Emery-Dreifuss muscular dystrophy (EDMD). This condition involves muscle weakness and cardiac issues highlighting the importance of Nesprin1 in muscle tissue integrity. Additionally Nesprin1 has been linked to cardiomyopathies where its interaction with proteins like emerin and lamin A/C is important for maintaining cardiac muscle health. Alterations in these interactions can lead to disrupted nuclear architecture and contribute to the pathogenesis of these disorders.