Abcam, ab192240, Anti-SMUG1 antibody [EPR15624]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal SMUG1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 10 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15624,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMUG1 also known as Single-strand-selective Monofunctional Uracil-DNA Glycosylase 1 plays a role in DNA repair mechanisms. It is an enzyme involved in the base excision repair (BER) pathway specializing in the removal of uracil from single-stranded DNA. SMUG1 has a molecular weight of about 33 kDa. The protein is expressed in various tissues with notable presence in the brain liver and lung. Its expression levels adjust according to the tissue's need for active DNA repair.
Biological function summary
The process of DNA metabolism relies heavily on mechanisms related to the SMUG1 enzyme. As part of the DNA repair machinery it identifies and excises uracil bases from DNA which result from deamination of cytosine or misincorporation during DNA synthesis. SMUG1 does not function within a larger protein complex but interacts dynamically with other repair proteins like AP endonucleases. This activity helps maintain genomic stability by preventing mutations that could arise from uracil incorporation.
Pathways
SMUG1 contributes importantly to the base excision repair pathway an important process for DNA maintenance. This pathway addresses small non-helix-distorting base lesions such as deaminated oxidized or alkylated bases. Within this pathway SMUG1's activity aligns with other glycosylases and the APEX1 protein which processes the abasic sites left after SMUG1 removes uracil. Furthermore SMUG1's function supports the overall cellular DNA damage response signaling when repair and further processing are necessary.
Alterations in the SMUG1 protein have connections to cancer and neurodegeneration. Loss of SMUG1 activity may result in accumulation of mutations due to unprocessed uracil in the DNA contributing to tumorigenesis. In particular studies have shown links between SMUG1 and glioma where defective DNA repair mechanisms escalate mutation rates. Also in neurodegenerative disorders it contributes directly through genomic instability with connections to APEX1 showing potential overlapping repair functions critical for neuron maintenance.