Abcam, ab192846, Anti-MAT1A + MAT2A antibody [EPR19048]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal MAT2A antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19048,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MAT1A and MAT2A are enzymes also known as methionine adenosyltransferase 1A and 2A which play a critical role in the synthesis of S-adenosylmethionine (SAMe). MAT1A has a molecular mass of about 42 kDa while MAT2A varies slightly due to post-translational modifications. These enzymes are mainly expressed in the liver and extrahepatic tissues respectively. MAT1A predominantly functions in the liver where it serves an essential part in maintaining normal liver metabolism.
Biological function summary
Methionine adenosyltransferase 1A and 2A function to catalyze the transfer of an adenosyl group from ATP to methionine forming SAMe. This reaction is part of a critical metabolic process acting as a source of methyl groups for methylation reactions. MAT1A commonly works as part of a homotetrameric complex while MAT2A can form both dimers and tetramers. This flexibility in complex formation helps regulate the production of SAMe based on cellular demands linking it to vital biomolecules synthesis.
Pathways
Methionine salvage pathways and polyamine biosynthesis include the significant involvement of MAT1A and MAT2A. These enzymes provide the necessary SAMe for the transmethylation and transsulfuration pathways connecting to other proteins such as GNMT and SAH hydrolase. The enzymes' activity influences the levels of important intermediates impacting these pathways and resulting metabolic outputs.
Abnormal expression or dysfunction of methionine adenosyltransferase 1A and 2A associates with liver cirrhosis and hepatocellular carcinoma. Alterations in the enzymes’ activity can disrupt normal SAMe levels affecting methylation reactions and subsequent gene expression patterns. Key proteins like cyclin-dependent kinase (CDK) also interact with these enzymes further linking them to cancer progression and outcomes.