Abcam, ab192998, Anti-ORP-3 antibody

Size: 100µg
Rabbit Polyclonal ORP-3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human OSBPL3 aa 450-550.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human OSBPL3 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.Q9H4L5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab192998 was affinity purified using an epitope specific to ORP-3 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ORP-3 also known as Oxysterol-binding protein-related protein 3 is a member of the oxysterol-binding protein family. It has a mass of approximately 100 kDa and is broadly expressed in a variety of tissues including the liver and spleen. ORP-3 contains a pleckstrin homology domain and an oxysterol-binding domain enabling its interactions with lipids and membranes. These structural aspects allow ORP-3 to have a role in lipid transport and cellular signaling.
Biological function summary
The protein contributes significantly to the regulation of lipid metabolism and cellular homeostasis. It is part of a complex that modulates lipid transport and signaling pathways affecting cellular growth and proliferation. ORP-3's interaction with phosphoinositides is particularly important for maintaining membrane integrity and function. These activities can influence cell movement and positioning which have implications in cellular responses to external stimuli.
Pathways
The protein interacts with several key pathways including the phosphoinositide signaling pathway and the sterol regulatory element-binding protein (SREBP) pathway. In the phosphoinositide pathway ORP-3 interacts with proteins like phosphatidylinositol 4-phosphate to facilitate lipid transport processes. In the SREBP pathway it indirectly influences cholesterol and fatty acid metabolism. These pathways position ORP-3 as an integral component in lipid homeostasis and signaling networks.
Disruptions in ORP-3 function link to lipid-related diseases such as atherosclerosis and certain cancers. In atherosclerosis ORP-3 contributes to cholesterol metabolism potentially affecting plaque formation. Its role in cancers involves its impact on cell proliferation and migration with connections to proteins such as Akt involved in cellular survival pathways. Understanding these associations helps in targeting ORP-3 for therapeutic interventions in these conditions.