Abcam, ab193555, Anti-Vimentin antibody [EPR3776] - BSA and Azide free

Size: 100µg / 1mg
Anti-Vimentin antibody [EPR3776] - BSA and Azide free (ab193555) is a rabbit recombinant monoclonal antibody for Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF, mIHC . Suitable for African green monkey, Human, Mouse, Rat . - KO validated for confirmed specificity - BSA, sodium azide, and glycerol-free for easy conjugation - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3776,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human, African green monkey,
Applications:IHC-P, Flow Cyt (Intra), WB, ICC/IF, mIHCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-Vimentin antibody [EPR3776] - BSA and Azide free (ab193555) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in African green monkey, Human, Mouse, Rat samples.
What is the molecular weight of Vimentin?
Anti-Vimentin [EPR3776] - BSA and Azide free (ab193555) specifically detects a band for Vimentin (UniProt: P08670) at a molecular weight of 54kDa.
Trusted by the scientific community
Anti-Vimentin [EPR3776] - BSA and Azide free (ab193555) was first used in a scientific publication in 2014 and has been cited over 40 times in peer-reviewed journals.
Specificity confirmed
The specificity of Anti-Vimentin antibody [EPR3776] - BSA and Azide free (ab193555) has been confirmed by Immunocytochemistry/ Immunofluorescence testing in VIM Knockout HeLa cells.
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, Carrier free - ab193555, HRP -
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, Alexa Fluor® 647 -
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, Alexa Fluor® 568 -
ab202504
, Alexa Fluor® 555 -
ab203428
, PE -
ab209446
, Alexa Fluor® 405 -
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ab314250
ab316214
, Carrier free -
ab316215
, Alexa Fluor® 750 -
ab321763
ab323238
, Carrier free -
ab323247
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze