Abcam, ab193717, Mouse beta IGH3 ELISA Kit

Size: 1 x 96Tests
Mouse beta IGH3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse beta IGH3 in Mouse in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 3 pg/mL,
Range:3 - 1000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Abcam's beta IGH3 mouse ELISA Kit (ab193717) is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of mouse BIGH3 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse beta IGH3 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures beta IGH3 present in the samples. The wells are washed and biotinylated anti-mouse beta IGH3 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of beta IGH3 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C