Abcam, ab193736, Rat beta NGF ELISA Kit

Size: 1 x 96Tests
Rat beta NGF ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat beta NGF in Rat in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Cell Lysate,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 15 pg/mL,
Range:15 - 15000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Abcam's beta NGF Rat ELISA Kit (ab193736) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat beta NGF in cell lysate and tissue lysate.
This assay employs an antibody specific for rat beta NGF coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures beta NGF present in the samples. The wells are washed and biotinylated anti-rat beta NGF antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of beta NGF bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta nerve growth factor (beta-NGF) is a significant protein that functions in the development and maintenance of the nervous system. It is also known as NGF or NGFB and has a molecular mass of around 26 kDa. This protein is highly expressed in a wide range of tissues including the brain skin and various glands. Researchers often use assays like growth factor assays and NGF ELISA kits to measure beta-NGF levels given its importance in several physiological processes.
Biological function summary
Beta nerve growth factor plays an important role in the growth survival and differentiation of neurons. As a member of the neurotrophin family it binds to receptors like TrkA and p75NTR on the surface of nerve cells. This binding activity helps to promote neuronal health and function especially in the peripheral and central nervous systems. While beta-NGF traditionally acts independently it also interacts with other signaling molecules to trigger cascades that support cell survival and neural connections.
Pathways
Beta-NGF prominently participates in the MAPK/ERK and PI3K/AKT signaling pathways. These pathways are essential for transmitting signals from the cell surface to the DNA in the nucleus therefore regulating gene expression linked to cell growth and survival. In these contexts beta-NGF works closely with proteins like Akt and ERK enhancing neuron resilience and promoting restorative mechanisms across the nervous system.
Beta nerve growth factor has been connected to neurodegenerative diseases like Alzheimer's disease and chronic pain conditions. Alterations in beta-NGF expression or signaling can impact neuronal survival contributing to the progression of these disorders. In Alzheimer's disease for example changes in NGF interactions with proteins like amyloid-beta and tau may exacerbate neurodegeneration. Similarly in pain syndromes disruptions in beta-NGF signaling can influence pain perception through pathways involving other neurotrophic factors.