Abcam, ab19534, Anti-Glutathione antibody [D8]

Size: 100µg
Anti-Glutathione antibody [D8] (ab19534) is a mouse monoclonal antibody detecting Glutathione in Flow Cytometry, ICC/IF . - Over 40 publications - Trusted since 2007
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:D8,
Isotype:IgG2a,
Carrier free:No,
Applications:ICC/IF, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
Product Specifications
Anti-Glutathione antibody [D8] (ab19534) is a mouse monoclonal antibody and is validated for use in Flow Cyt, ICC/IF in species independent samples.
Anti-Glutathione antibody [D8] (ab19534) specifically detects Glutathione (UniProt ID: ; Molecular weight: kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Glutathione antibody [D8] (ab19534) has high sensitivity and specificity.
Anti-Glutathione antibody [D8] (ab19534) has been cited over 46 times in peer reviewed journals and is trusted by the scientific community.
Anti-Glutathione antibody [D8] (ab19534) has 10 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.6Preservative: 0.01% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glutathione often referred to as GSH is a small tripeptide with a molecular mass of approximately 307.33 g/mol. It is composed of amino acids glutamine cysteine and glycine. Glutathione is found in almost all cells with high concentrations especially in the liver. As a powerful antioxidant it functions by reducing reactive oxygen species (ROS) and maintaining the redox status of cells. Glutathione exists in two forms: reduced (GSH) and oxidized (GSSG). The balance between these forms known as the GSH/GSSG ratio is critical for cellular health and is commonly assessed using a glutathione assay kit.
Biological function summary
Glutathione plays an important role in detoxification processes protecting cells from damage caused by free radicals peroxides and heavy metals. It acts as a substrate for various enzymes like glutathione peroxidase and glutathione S-transferase involved in neutralizing oxidative damage. Glutathione doesn't typically form a complex but it does participate in disulfide bond exchange reactions which are critical in maintaining cellular protein functions and structures.
Pathways
Glutathione is integral to cellular antioxidant defense and phase II detoxification pathways. It closely interacts with proteins like glutathione peroxidase in the reduction of hydrogen peroxide to water. Also glutathione's role in the pentose phosphate pathway supports the regeneration of NADPH which is essential for maintaining the reduced form of glutathione. This interplay is important for protecting cells against oxidative stress and ensuring cellular energy is balanced.
Glutathione has significant implications in conditions such as Parkinson's disease and liver cirrhosis. In Parkinson's disease researchers observe lower levels of glutathione in affected brain regions suggesting an important role in neurodegeneration. Glutathione levels also impact liver health with deficiencies leading to ineffective detoxification and the potential for liver damage or cirrhosis. In these contexts glutathione interacts with proteins involved in redox balance and neurotransmitter regulation emphasizing its broad significance in maintaining human health.