Abcam, ab196048, Recombinant human CDK5 + p25 protein

Size: 10µg
Recombinant human CDK5 + p25 protein is a Human Full Length protein, in the 99 to 307 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific Activity: 312 pmol/min/μgAssay was done in a Kinase buffer using Histone H1 as a substrate with 20 μM ATP at 30°C for 30 min. The amount of ATP transferred was calculated using a kinase reagent,
Accession:Q00535,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.12% Glutathione, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cyclin-dependent kinase 5 (Cdk5) is a type of protein kinase known mainly for its regulatory role in the nervous system. Cdk5 is activated upon binding to its activators p35 or p39 proteins or in some cases to a truncated version known as p25. The Cdk5/p35 complex plays a role in cellular signaling pathways with p35 having a molecular mass of approximately 35 kDa. Cdk5 is expressed in post-mitotic neurons but also found in other cells under specific conditions. These proteins maintain structural and functional stability within cells where they are expressed contributing to multiple physiological processes.
Biological function summary
Cdk5 with its activator forms a complex that is essential for neural development and function. The Cdk5/p35 or Cdk5/p25 complexes participate in neuron migration and axon guidance. When p35 cleaves into p25 it can lead to prolonged activation of Cdk5 causing dysregulation of cellular processes. This dysregulation affects cytoskeletal dynamics leading to alterations in synaptic function and signal transmission in neurons. Therefore Cdk5 helps cells to manage normal and necessary development processes in the brain and neurons.
Pathways
Cdk5 and its complexes influence Ca²⁺-dependent signaling pathways and are integral to the regulation of neurotransmitter release. The Ras-related protein Rab-3A and protein phosphatase PP1 are among proteins interacting with Cdk5 through signaling cascades. These connections highlight Cdk5's involvement in regulating synaptic vesicle exocytosis and cytoskeletal integrity. Such pathways demonstrate the importance of Cdk5 in maintaining neuronal functionality and connectivity.
Cdk5 particularly in its Cdk5/p25 form is linked to Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In these disorders the aberrant activation of Cdk5 leads to tau hyperphosphorylation and neurofibrillary tangle formation events associated with Alzheimer's-related neurodegeneration. In ALS Cdk5 dysregulation contributes to motor neuron damage. The alteration from p35 to p25 is a critical factor in these pathologies interacting with tau protein which is connected to the progression of these degenerative conditions.