Abcam, ab196558, Anti-APRT antibody

Size: 100µL
Rabbit Polyclonal APRT antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Adenine phosphoribosyltransferase.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human Adenine phosphoribosyltransferase.P07741

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Adenine phosphoribosyltransferase (APRT) also known as APTR is an enzyme involved in the purine salvage pathway. It has a molecular mass of approximately 23 kDa and gets expressed in numerous tissues including the liver kidney and brain. This protein catalyzes the conversion of adenine and phosphoribosyl pyrophosphate (PRPP) to adenosine monophosphate (AMP) facilitating the reutilization of adenine into nucleotides which is necessary for DNA and RNA synthesis.
Biological function summary
Adenine phosphoribosyltransferase plays a role in cellular nucleotide pool maintenance by rescuing adenine from degradation and incorporating it back into cellular metabolism. This enzyme does not form part of a larger enzyme complex but acts independently to perform its catalytic function. Properly maintaining nucleotide balance is fundamental for cellular activities and imbalances can lead to cellular stress or DNA damage responses.
Pathways
The purine salvage pathway is where adenine phosphoribosyltransferase functions critically. This pathway operates alongside the de novo synthesis of purine nucleotides working to conserve energy and resources during nucleotide synthesis. APRT interacts closely with hypoxanthine-guanine phosphoribosyltransferase (HGPRT) within this salvage process to recycle purines effectively reducing the need for energy-expensive de novo synthesis.
Defects in the adenine phosphoribosyltransferase gene can result in the condition known as APRT deficiency. This genetic disorder leads to the buildup of 28-dihydroxyadenine causing nephrolithiasis and potential kidney damage. APRT's function connects with xanthine oxidase activity influencing uric acid metabolism. The accumulation of poorly soluble adenine derivatives can also cause crystalluria and can influence uric acid levels connecting APRT to the pathophysiology of gout in some cases.