Abcam, ab196562, Anti-UPD antibody

Size: 100µL
Rabbit Polyclonal UPD antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UROD.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human UROD.P06132

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ubiquitously expressed UDP-glucuronosyltransferase protein family often referred to as UGTs plays essential roles in the detoxification and metabolism of both endogenous and exogenous compounds. Individual UGT enzymes convert lipophilic molecules into more water-soluble glucuronides facilitating their excretion. This process involves the transfer of glucuronic acid from uridine diphosphate glucuronic acid (UDPGA) to substrates. UGTs display diverse tissue expression with significant activity in the liver gastrointestinal tract and kidneys. These enzymes typically have a molecular mass of around 50-60 kDa.
Biological function summary
UDP-glucuronosyltransferases are integral to phase II metabolism transforming small hydrophobic molecules for elimination. The UGT family functions within the microsomal enzyme system and associates with lipid membranes in the endoplasmic reticulum. UGTs work as monomers or form enzyme complexes particularly with other phase II enzymes such as sulfotransferases to efficiently manage the body's biochemical landscape. They recognize a wide range of substrates including hormones bile acids and drugs highlighting their metabolic versatility.
Pathways
UDP-glucuronosyltransferases participate primarily in the detoxification pathway. This pathway relies on UGTs for the conjugation of xenobiotics aiding in detoxifying harmful compounds. The enzymes also integrate into the bilirubin metabolic pathway where they catalyze the conjugation of bilirubin reducing its toxicity. Notably UGT1A1 a member of the UGT family takes part in this pathway and interacts with other proteins such as cytochrome P450 enzymes which are important in drug metabolism and synthesis of cholesterol steroids and other lipids.
Defects or deficiencies in UDP-glucuronosyltransferases can lead to clinical conditions like Gilbert's syndrome and Crigler-Najjar syndrome. Gilbert's syndrome results from mutations in UGT1A1 causing mild unconjugated hyperbilirubinemia. Crigler-Najjar syndrome a more severe disorder arises from significantly impaired UGT1A1 function leading to extreme cases of hyperbilirubinemia that may require liver transplantation. The interrelationship between UGT1A1 and proteins like albumin which binds bilirubin in the bloodstream highlights the important impact of glucuronidation in maintaining bilirubin homeostasis.