Abcam, ab197547, Anti-Ki67 antibody [SP6] - BSA and Azide free

Size: 100µg / 1mg
Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Ki67 in Western Blot, Flow Cytometry, IHC-P, ICC/IF, mIHC . Suitable for Human, Mouse, Rat . - Clone SP6 is the most cited clone to Ki67 - KO validated for confirmed specificity - BSA, sodium azide, and glycerol-free for easy conjugation - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP6,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, mIHC, WB, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:C-terminus,
Specificity:Ki67 is mainly expressed in proliferating cells. For normal tissue samples (e.g., liver, kidney), no staining may be typically observed due to low level of proliferation and little expression of Ki67. For malignant tissue samples (e.g., colon carcinoma, breast carcinoma), it is more easily to find Ki67 in the proliferating cells of these tissues (PMID: 6206131, 10653597, 34183782).FURTHER INFORMATION ON SPECIFICITY(Chinese Version)

Product details:
What is this antibody validated in?
Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in Human, Mouse, Rat samples.
What is the molecular weight of Ki67?
Anti-Ki67 [SP6] - BSA and Azide free (ab197547) specifically detects a band for Ki67 (UniProt: P46013) at a molecular weight of 358kDa.
Collaboration
Anti-Ki67 [SP6] - BSA and Azide free (ab197547) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).
Specificity confirmed
The specificity of Anti-Ki67 antibody [SP6] - BSA and Azide free (ab197547) has been confirmed by Immunocytochemistry/ Immunofluorescence testing in MKI67 Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [SP6] also available for your convenience:
ab16667
ab21700
, Carrier free - ab197547,
ab231172
, ab238624, Oligonucleotide - ab279361, Alexa Fluor® 488 -
ab281847
, Alexa Fluor® 647 -
ab281928
, PE -
ab282173
, APC -
ab314285
ab320709
, Carrier free -
ab320710
, ab323292
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.
Biological function summary
The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.
Pathways
Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.
Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.