Abcam, ab197749, Canine CKM ELISA Kit

Size: 1 x 96Tests
Canine CKM ELISA Kit is a single-wash 90-min Simplestep used to quantify Canine CKM with a sensitivity of 20 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Tissue Extracts, Serum,
Reacts with:Dog,
Assay type:Sandwich (quantitative),
Sensitivity:= 20 pg/mL,
Range:0.08 - 5 ng/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Canine CKM ELISA Kit (ab197749) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CKM protein in serum and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Canine CKM with 20 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Creatine kinase (CK) catalyzes creatine to phosphocreatine (PCr) by consuming adenosine triphosphate (ATP) and producing adenosine diphosphate (ADP). This process is reversible, thus PCr and ADP can be used to generate ATP and creatine. There are three known cytoplasmic isoenzymes (CKMM- skeletal muscle, CKMB-myocardium, and CKBB- nervous tissue). Phosphocreatine serves as an energy storage for rapid regeneration of ATP. Creatine is used in tissues with high energy demands, such as skeletal muscle, heart, and brain. Expression of CKM in serum are based on muscle mass, age, gender, race, and physical activity. Skeletal muscle expresses approximately 98% CKMM and 1% CKMB, myocardium expresses 70% CKMM and 25-30% CKMB, and neural tissue expresses a majority of CKBB.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols