Abcam, ab197925, Anti-PICH antibody

Size: 100µL
Rabbit Polyclonal PICH antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ERCC6L.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ERCC6L. The exact immunogen used to generate this antibody is proprietary information.Q2NKX8

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein PICH also known as Plk1-interacting checkpoint helicase is an important factor in maintaining chromosome integrity during cell division. PICH is a helicase with a molecular mass of approximately 135 kDa. This protein is mainly expressed in tissues with a high rate of cell division. It possesses ATPase activity which allows it to unwind DNA structures playing a mechanical role in chromosome segregation. The helicase activity of PICH is necessary in mitosis where it interacts with centromeres during spindle assembly checkpoint activation.
Biological function summary
PICH contributes to the resolution of ultra-fine DNA bridges which occur during the late stages of mitosis. These DNA bridges can compromise genome stability leading to genetic disorders if not properly resolved. PICH works with other proteins like topoisomerase II and Bloom syndrome protein (BLM) to manage these structures. This activity suggests PICH is part of a complex necessary for protecting chromosomal stability during cell division ensuring accurate segregation of sister chromatids.
Pathways
PICH plays a role in the spindle assembly checkpoint (SAC) and mitotic exit pathway. Within these pathways PICH interacts with proteins such as Polo-like kinase 1 (Plk1) and Aurora B both important for the regulation of mitosis. PICH's interaction with these proteins highlights its significance in controlling the proper timing and execution of chromosome separation which is essential for correct mitotic progression and prevention of aneuploidy.
PICH is associated with conditions that involve genomic instability like cancer. Aberrations in PICH function can result in incorrect chromosome segregation contributing to tumorigenesis. Additionally PICH is linked with disorders involving defective DNA repair mechanisms such as those involving BRCA1 a protein important for repairing DNA double-strand breaks. Dysfunctional PICH leads to enhanced chromosomal aberrations highlighting its relevance in maintaining genomic integrity and linking it to significant pathological states.