Abcam, ab198595, Alexa Fluor® 488 Anti-TRF2 antibody [EPR3517(2)]

Size: 100µL
Rabbit Recombinant Monoclonal TRF2 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3517(2),
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRF2 also known as Telomeric Repeat-binding Factor 2 is a protein important for telomere protection and maintenance. TRF2 weighs approximately 55 kDa and is widely expressed in human tissues especially in those with high turnover rates. This protein binds specifically to double-stranded telomeric DNA and forms a protective cap at the end of chromosomes helping to regulate telomere length and integrity. By securing the telomere's structure TRF2 prevents chromosomes from being recognized as damaged DNA.
Biological function summary
TRF2 plays a central role in telomere function by assembling into the shelterin complex. This complex which includes other proteins like TRF1 TIN2 POT1 TPP1 and RAP1 is essential for controlling telomere length and stability. TRF2 is responsible for inhibiting DNA damage response pathways at telomeres ensuring that the natural chromosomal ends are not mistaken for double-strand breaks. This suppression of unwanted DNA damage response is important for normal cell division and avoiding cellular senescence.
Pathways
Researchers have placed TRF2 within the DNA repair and telomere maintenance pathways. TRF2 interacts with several other proteins such as Ku70/80 and RAD51 that are instrumental in the DNA repair process. Furthermore TRF2 influences the Alternative Lengthening of Telomeres (ALT) pathway which is a telomere lengthening process found in some cancer cells. These connections highlight TRF2's role in maintaining genomic stability through its capacity to regulate telomere-associated proteins and pathways.
TRF2 has been linked to cancer and aging. Elevated levels of TRF2 have been detected in various cancers including breast and prostate cancer suggesting a role in tumorigenesis where it contributes to the stability of cancer cell telomeres. Additionally defects in TRF2 or its interaction with partner proteins like POT1 can lead to dysfunctional telomeres which contribute to the aging process and age-related diseases by affecting cell proliferation and survival. These links demonstrate the importance of TRF2 in normal cellular function and its potential as a therapeutic target in disease intervention.