Abcam, ab199083, Mouse Lipocalin-2 ELISA Kit (NGAL)

Size: 1 x 96Tests / 10 x 96Tests
Mouse Lipocalin-2 ELISA Kit (NGAL) is a single-wash 90-min Simplestep used to quantify Mouse Lipocalin-2 (NGAL) with a sensitivity of 1.1 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 10x96 plates for bulk orders - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 15 citations
Key facts
Detection method:Colorimetric,
Sample types:Urine, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.1 pg/mL,
Range:4.69 - 300 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse Lipocalin-2 ELISA Kit (NGAL) ab199083 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Lipocalin-2 (NGAL)in cell culture supernatant, citrate plasma, EDTA plasma, serum, urine. This SimpleStep sensitivity is 1.1 pg/mL.
How the assay works
Mouse Lipocalin-2 (NGAL)SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse Lipocalin-2 ELISA Kit (NGAL) ab199083 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Lipocalin-2 (also known as Neutrophil gelatinase-associated lipocalin or NGAL) is an iron binding and iron trafficking protein. Lipocalin-2 is involved in multiple cellular processes including apoptosis, innate immunity and renal development. Mice deficient in Lipocalin-2 appear normal but have increased susceptibility to bacterial infection. The bacteriostatic function may be related to Lipocalin-2 limiting bacterial iron supply. Mouse Lipocalin-2 has 81% and 62% protein sequence identity to rat and human Lipocalin-2, respectively.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C