Abcam, ab199225, Alexa Fluor® 488 Anti-Ku70 antibody [EPR4027]

Size: 100µL
Rabbit Recombinant Monoclonal Ku70 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4027,
Isotype:IgG,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ku70 also known as XRCC6 or K331 is a DNA repair protein with a molecular mass of approximately 70 kDa. It belongs to the Ku protein family which includes Ku80. This protein expresses mainly in the nucleus of cells across various tissue types. As a part of a heterodimer together with Ku80 Ku70 forms the Ku70/Ku80 complex which binds to free DNA ends allowing DNA repair processes to occur efficiently.
Biological function summary
The Ku70 protein plays a significant role in maintaining genomic stability. It is an important component of the non-homologous end joining (NHEJ) pathway a primary mechanism for repairing double-strand breaks in DNA. By forming the Ku70/Ku80 complex it accurately aligns DNA ends to facilitate repair and prevent chromosomal abnormalities. The Ku70 protein also involves in telomere maintenance and can influence cell cycle regulation.
Pathways
Ku70 contributes mainly to the NHEJ DNA repair pathway. This pathway is important for cellular response to DNA damage and involves other proteins such as DNA-PKcs (DNA-dependent protein kinase catalytic subunit) and XRCC4. Ku70's interaction with DNA-PKcs enables the activation of its kinase activity which is essential for the repair process. Additionally Ku70 is involved in the homologous recombination pathway where it interacts with proteins like Rad51 to ensure repair fidelity.
Research links the Ku70 protein to cancer and neurodegenerative diseases. Dysregulation or mutations affecting Ku70 can lead to genomic instability contributing to cancer development. Ku70 also shows connections to neurodegenerative conditions like Alzheimer's disease where its role in DNA repair and apoptosis regulation may impact neuronal cell survival. Interactions with p53 a protein important for DNA damage response further underline Ku70's influence in these disease contexts.