Abcam, ab20034, Anti-FOXP3 antibody [236A/E7]

Size: 20µg / 100µg / 1mg
Anti-FOXP3 antibody [236A/E7] (ab20034) is a mouse monoclonal antibody detecting FOXP3 in Western Blot, IHC-P, mIHC . Suitable for Human . - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Over 590 publications - Trusted since 2005
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:236A/E7,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, mIHCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The epitope recognized by FOXP3 antibody [236A/E7] (ab20034) is between amino acids 105-236. FOXP3 antibody [236A/E7] (ab20034) is expected to detect full length FOXP3 as well as both cleaved forms.

Product details:
Anti-FOXP3 antibody [236A/E7] (ab20034) is a mouse recombinant monoclonal antibody and is validated for use in IHC-P, WB and mIHC.
Anti-FOXP3 antibody [236A/E7] (ab20034) was first used in a scientific publication in 2005 and has been cited over 593 times in peer reviewed journals. It's performance in IHC in human samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-FOXP3 antibody [236A/E7] (ab20034) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-FOXP3 antibody [236A/E7] (ab20034) has 37 independent reviews from customers.
Anti-FOXP3 antibody [236A/E7] (ab20034) specifically detects FOXP3 (UniProt ID: Q9BZS1; Molecular weight: 48kDa) and is sold in a convenient trial size to enable initial testing (10ug, 20 µg) and larger sizes for subsequent scaling up experiments (100 µg and 1 mg).
Conjugation-ready, carrier free format available for antibody clone 236A/E7 -
ab96048
FOXP3 is essential for the development and function of regulatory T cells (Tregs), which help maintain immune tolerance. In cancer, FOXP3-expressing Tregs suppress anti-tumor immune responses, aiding tumor growth and progression.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FOXP3 also known as Forkhead box P3 is a protein weighing approximately 47 kDa. This protein acts as a transcription factor and is essential for the development and function of regulatory T cells (Tregs). FOXP3 is predominantly expressed in these cells and is critical for their role in immune system regulation. Regulatory T cells prevent autoimmune responses by maintaining tolerance to self-antigens. Mouse models demonstrate similar expression patterns and researchers often study FOXP3 using techniques like immunohistochemistry (IHC) and flow cytometry to understand its presence and function in tissue samples.
Biological function summary
The regulatory role of FOXP3 in T cells influences the immune response. It is a part of the Treg cell lineage and serves by modulating cytokine expression. In particular FOXP3 contributes to the suppression of effector T cell functions mediating immune homeostasis. Without FOXP3 Treg cells lose their function and ability to regulate immune responses effectively. The complexity of Treg cell function is partly attributed to the multitude of interactions FOXP3 facilitates within cell signaling pathways. In research scientists frequently utilize FOXP3 antibodies like 236A/E7 and 3G3 to study its expression and function in various biological contexts.
Pathways
FOXP3 integrates into the regulatory framework of pathways such as the TGF-beta signaling and IL-2 signaling pathways. These pathways are important for Treg differentiation and regulatory capacity. FOXP3 interacts with the signaling molecules and co-factors within these pathways including SMAD3 in the TGF-beta pathway aligning with its role in Treg functionality. FOXP3 functions alongside proteins like RUNX1 and CTLA-4 which further modulates its regulatory effects on T cell activity. These interactions illustrate FOXP3's extensive reach within immune regulation and development.
FOXP3 has associations with immune-related conditions notably autoimmune disorders and inflammatory diseases. Mutations or deficiencies in FOXP3 expression often lead to conditions like IPEX syndrome a rare autoimmune disorder. In cancer altered FOXP3 activity within tumor-infiltrating lymphocytes can influence tumor progression and immune evasion. In these contexts FOXP3 interacts with proteins such as IL-10 and TGF-beta to modulate immune responses potentially altering disease outcomes. Understanding FOXP3's involvement in these diseases provides insight into therapeutic targets and interventions.