Abcam, ab200382, Anti-NEMF antibody

Size: 100µL
Rabbit Polyclonal NEMF antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NEMF.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NEMF. The exact immunogen used to generate this antibody is proprietary information.O60524

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NEMF protein also known as NEMF Shipping plays an important role in cellular processes. It functions as an important component of the ribosome-associated quality control complex facilitating the identification and degradation of faulty nascent peptides. NEMF has a molecular mass of approximately 110 kDa and is mostly expressed in the cytoplasm of eukaryotic cells. This protein operates with other factors to maintain the integrity of protein synthesis and prevent the accumulation of malfunctioning proteins within the cell.
Biological function summary
NEMF is part of the ribosome-associated quality control complex that monitors and ensures proper translation and protein folding. This complex attaches to the ribosome during translation stalling events marking incomplete or misfolded polypeptides for ubiquitination and subsequent degradation by the proteasome. By participating in this surveillance mechanism NEMF supports protein homeostasis which is important for normal cellular function. It often collaborates with other components like LTN1 an E3 ubiquitin ligase and RQC2/NEM2 aiding efficient processing of aberrant proteins.
Pathways
The NEMF protein has substantial importance in the cellular quality control pathways. It engages intimately with the ribosome-associated quality control pathway which rescues stalled ribosomes and targets them for recycling. Additionally NEMF is involved in pathways that regulate protein synthesis at times of cellular stress. Within these frameworks NEMF partners with proteins like RPL26 and ASCC3 which work to maintain translational fidelity and proteostasis.
NEMF has been implicated in neurodegenerative diseases and cancer. Abnormalities in the quality control mechanisms it safeguards can lead to the accumulation of defective proteins contributing to conditions such as amyotrophic lateral sclerosis (ALS). Furthermore altered NEMF function or expression has been linked to tumorigenesis as proper protein synthesis and degradation play significant roles in cancer progression. NEMF works alongside other proteins like p53 which is often mutated in cancer highlighting its involvement in pathways important for cellular health and disease prevention.