Abcam, ab201792, Anti-NLRC4 antibody [EPR19733]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal NLRC4 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 26 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19733,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NLRC4 also known as CARD12 or IPAF is an intracellular protein that plays an important role in inflammation and immune responses. It belongs to the NOD-like receptor (NLR) family specifically the NLRC subfamily and contains a caspase activation and recruitment domain (CARD). NLRC4 weighs approximately 120 kDa and is expressed abundantly in macrophages and dendritic cells. It participates mechanically by recognizing cytosolic pathogen-associated molecular patterns (PAMPs) and activating the immune response.
Biological function summary
NLRC4 forms part of the inflammasome complex which is a multi-protein unit essential for innate immunity. This protein helps detect and respond to bacterial components enabling the activation of caspase-1. Caspase-1 then processes pro-inflammatory cytokines like interleukin-1β and interleukin-18 into their active forms. This mechanism helps defend against bacterial infections and maintain tissue homeostasis.
Pathways
NLRC4 functions within the inflammasome activation pathway and intersects with autophagy processes. It facilitates the detection of intracellular flagellin through its interaction with the NAIP proteins leading to the activation of caspase-1. NLRC4's activity results in inflammasome assembly which links it to both the innate immune response and cell death pathways. This association positions it near proteins such as ASC which acts as an adapter molecule in inflammasomes.
NLRC4 is linked to autoinflammatory conditions and gastrointestinal diseases. Mutations or dysregulation in NLRC4 may result in the development of autoinflammatory syndromes characterized by recurrent fever and systemic inflammation. These conditions associate with other proteins in the inflammasome complex like NLRP3 which also mediates inflammatory responses. Furthermore disturbances in NLRC4 function can contribute to digestive system diseases emphasizing its relevance in maintaining balanced immune activity.